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Anti-CD3 antibody [CD3-12]
Anti-CD3 antibody [CD3-12] from Abcam
Be the first to write a review!
Citations:
(79)
Supplier Page
Supplier Page from
Abcam for
Anti-CD3 antibody [CD3-12]
Product Specs
Company Info
Citations
Reviews
Videos
Product Specs
Item
Anti-CD3 antibody [CD3-12]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab11089
Quantity
100 UG
Applications
Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/Tag
Unconjugated
Format
Purified IgG prepared from tissue culture supernatant
Isotype
IgG1
Clone
CD3-12
Host
Rat
Antibody Type
Primary
Clonality
Monoclonal
Concentration
1 mg/ml
Research Area
Immuno-oncology
Gene Name
CD3E
Reactivity
Human, Mouse, Rat
Target
CD3
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (79)
(1)
Imaging and AI based chromatin biomarkers for diagnosis and therapy evaluation from liquid biopsies
NPJ Precis Oncol
December 14, 2023
Kiran Challa, Daniel Paysan, Dominic Leiser, Nadia Sauder, Damien C. Weber, G. V. Shivashankar
mouse anti-Lamin A/C from
Abcam
(catalog no.
ab8980
), monoclonal rat anti-CD3 from
Abcam
(catalog no.
ab11089
), recombinant Alexa Fluor 647 anti-CD8 alpha antibody from
Abcam
(catalog no.
ab196193
), monoclonal
(2)
Topical Calcipotriol Plus Imiquimod Immunotherapy for Nonkeratinocyte Skin Cancers
JID Innovations
November 1, 2023
Marjan Azin, Kenneth H. Ngo, Jennet Hojanazarova, Shadmehr Demehri
1 hour and stained with anti-
mouse
primary antibodies, including anti-CD3 antibody (catalog number
ab11089
,
Abcam
, Cambridge, United Kingdom) and anti-CD4 antibody (catalog number
ab183685
,
Abcam
) overnight
(3)
Novel miRNA-based drug CD5-2 reduces liver tumor growth in diethylnitrosamine-treated mice by normalizing tumor vasculature and altering immune infiltrate
Frontiers in Immunology
September 18, 2023
Ken Liu, Jinbiao Chen, Yang Zhao, Jade Boland, Ka Ka Ting, Glen Lockwood, Catriona McKenzie, James Kench, Mathew A. Vadas, Jennifer R. Gamble,
et al.
purchased from
Thermo Fisher Scientific
(Scoresby, VIC 3179, Australia). Antibodies against
CD3
(cat#
ab11089
),
CD34
(cat#
ab81289
) and carbonic anhydrase 9 (
CAIX
, cat#
ab184006
) were bought from
Abcam
(Cambridge,
(4)
The MIF promoter SNP rs755622 is associated with immune activation in glioblastoma
JCI insight
July 10, 2023
Alban TJ, Grabowski MM, Otvos B, Bayik D, Wang W, Zalavadia A, Makarov V, Troike K, McGraw M, Rabljenovic A,
et al
.
different sets of markers and indicated below: Set 1 (triple
immunofluorescence
staining
): DAPI, CD3 (
ab11089
,
Abcam
,
1:50
),
CD8
(
85336
,
Cell Signaling
,
1:50
),
CD107a
(
NBP2-52721
,
Novus
Bio,
1:50
); Set 2 (double
(5)
Perturbation of endoplasmic reticulum proteostasis triggers tissue injury in the thyroid gland
JCI insight
June 22, 2023
Zhang X, Young C, Liao XH, Refetoff S, Torres M, Tomer Y, Stefan-Lifshitz M, Zhang H, Larkin D, Fang D,
et al
.
(
ab109510
,
Abcam
); rabbit anti-
CD45
(
ab10558
,
Abcam
);
rat
anti-Mac2 (
14-5301-81
,
Invitrogen
); rat anti-CD3 (
ab11089
,
Abcam
);
rat
anti-
CD8a
(
14-0808-82
,
Invitrogen
);
rat
anti-
CD19
(
14-0194-82
,
Invitrogen
); rabbit anti-P62
(6)
Metronomic capecitabine inhibits liver transplant rejection in rats by triggering recipients’ T cell ferroptosis
World Journal of Gastroenterology
May 28, 2023
Hao Wang, Zheng-Lu Wang, Sai Zhang, De-Jun Kong, Rui-Ning Yang, Lei Cao, Jian-Xi Wang, Sei Yoshida, Zhuo-Lun Song, Tao Liu,
et al.
serum. Then the sections were incubated overnight at 4 ℃ with a rat anti-CD3 antibody (
CD3
-12, Cat#
ab11089
, RRID: AB_2889189;
Abcam
, Cambridge, MA, United States) and incubated with a secondary antibody (
Goat Anti-Rat IgG H&L
(7)
EphB4 monomer inhibits chronic graft vasculopathy in an aortic transplant model
JVS-Vascular Science
May 22, 2023
John T. Langford, Luis Gonzalez, Ryosuke Taniguchi, Anand Brahmandam, Weichang Zhang, Alan Dardik
hour at room temperature and then incubated overnight at 4°C with either CD68 (
ab31630
;
Abcam
) or
CD3
(
ab11089
). Sections were then treated with secondary antibodies at room temperature for 1 hour using Alexa Fluor
(8)
Rapid generation of a mouse model for evaluating on-target normal tissue toxicity of human CAR-T cells using replication-defective recombinant adenovirus
Journal of advanced research
May 1, 2023
Liao Q, Liu Z, Zhu C, He H, Feng M, Jiang L, Ding X, Sun R, Zhang X, Xu J.
CD47 (
ab218810
,
Abcam
)
for immunohistochemistry
(
IHC
), and immunostained with anti-human CD3 (Cat #:
ab11089
,
Abcam
), anti-FLAG tag (Cat #:
637301
,
Biolegend
) antibodies and
DAPI
for
immunofluorescence
, which
(9)
Selective emergence of antibody-secreting cells in the multiple sclerosis brain
eBioMedicine
March 1, 2023
Laurens Bogers, Hendrik J. Engelenburg, Malou Janssen, Peter-Paul A. Unger, Marie-José Melief, Annet F. Wierenga-Wolf, Cheng-Chih Hsiao, Matthew R.J. Mason, Jörg Hamann, Jamie van Langelaar,
et al.
incubated overnight with primary antibody (CD19 (rabbit),
1:200
, 11501, Biolegend; CD3 (
rat
),
1:50
,
ab11089
,
Abcam
; CXCR5 (
mouse
),
1:100
, MAB190, RD Systems). Endogenous peroxidase activity was quenched in a
(10)
CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance
Cancer immunology research
March 1, 2023
Lao L, Zeng W, Huang P, Chen H, Jia Z, Wang P, Huang D, Chen J, Nie Y, Yang L,
et al
.
(
Abcam
, #
ab17147
,
1:100
), rabbit-anti-human CD45 (
Abcam
, #
ab10558
,
1:100
), rat-anti-human CD3 (
Abcam
, #
ab11089
,
1:100
), rabbit-anti-human CD161 (Abcam, #ab259916,
1:50
),
mouse
-anti-
human
Vα7.2 (
BioLegend
, #
351702
,
(11)
Combined PD-1, BRAF and MEK inhibition in BRAF(V600E) colorectal cancer: a phase 2 trial
Nature medicine
February 1, 2023
Tian J, Chen JH, Chao SX, Pelka K, Giannakis M, Hess J, Burke K, Jorgji V, Sindurakar P, Braverman J,
et al
.
serum (Sigma-Aldrich) for 1 h at room temperature. The slides were incubated with anti-CD3 (
Abcam
;
ab11089
;
1:800
dilution) and anti-
CD8
(
Cell Signaling
;
98941
S;
1:400
dilution) primary antibodies overnight
(12)
Infection of SARS-CoV-2 causes severe pathological changes in mouse testis
Journal of genetics and genomics = Yi chuan xue bao
February 1, 2023
Chen M, Li S, Liu S, Zhang Y, Cui X, Lv L, Liu B, Zheng A, Wang Q, Duo S,
et al
.
SOX9
(Millipore,
AB5535
;
Sigma
,
AMAB90795
), Nucleoprotein (
Sino biological
,
40143-R001
), CD3 (
Abcam
,
ab11089
), 3β-HSD (
Santa Cruz
,
sc-30820
). The secondary antibodies were: FITC-conjugated donkey anti-rabbit IgG
(13)
The unforeseen intracellular lifestyle of
Enterococcus faecalis
in hepatocytes
Gut Microbes
December 31, 2022
Natalia Nunez, Aurélie Derré-Bobillot, Nicolas Trainel, Goran Lakisic, Alexandre Lecomte, Françoise Mercier-Nomé, Anne-Marie Cassard, Hélène Bierne, Pascale Serror, Cristel Archambaud
monoclonal anti-claudin 1 (Clinisciences, sc-166338 diluted
1:100
),
rat
monoclonal anti-CD3 (
Abcam
,
ab11089
diluted
1:100
) and
mouse
anti-Ly6G (BioLegend, France,
clone
A8; diluted
1:50
), followed by
staining
(14)
Cerebrospinal fluid immune dysregulation during healthy brain aging and cognitive impairment
Cell
December 22, 2022
Piehl N, van Olst L, Ramakrishnan A, Teregulova V, Simonton B, Zhang Z, Tapp E, Channappa D, Oh H, Losada PM,
et al
.
imaging We stained 5μm paraffin embedded brain tissue sections using antibodies rat anti-CD3 (
Abcam
ab11089
), rabbit anti-CXCR6 (Abcam ab273116),
mouse
anti-
CXCL16
(
Thermo
MA5–27845
), rabbit anti-amyloid-β (
Cell Signaling
(15)
Sympathetic innervation of human and porcine spleens: implications for between species variation in function
Bioelectronic Medicine
December 19, 2022
Logan G. Kirkland, Chloe G. Garbe, Joseph Hadaya, Paul V. Benson, Brant M. Wagener, Sanjin Tankovic, Donald B. Hoover
0.4% Triton X-100, 1% BSA, and two primary antibodies (rabbit anti-TH and mouse anti-CD3,
1:20
,
Abcam
ab11089
). The ImmPRESS Duet HRP/AP (alkaline phosphatase) Polymer Reagent included a mixture of HRP anti-rabbit
(16)
Thymic stromal lymphopoietin-stimulated CD4+ T cells induce senescence in advanced breast cancer
Frontiers in Cell and Developmental Biology
November 17, 2022
Margherita Boieri, Emanuela Marchese, Quan Minh Pham, Marjan Azin, Lauren E. Steidl, Anna Malishkevich, Shadmehr Demehri
immunofluorescence
(Guennoun et al., 2022)
staining
, sections were incubated with CD3 (catalog no.
Ab11089
,
Abcam
, Waltham, MA), cytokeratin [catalog no. M3515, Dako (Agilent), Santa Clara, CA, United States]
(17)
Cannabidiol markedly alleviates skin and liver fibrosis
Frontiers in Pharmacology
October 19, 2022
Carmen del RÃo, Francisco Ruiz-Pino, MarÃa E. Prados, Bernd L. Fiebich, Manuel Tena-Sempere, Eduardo Muñoz
with anti-TNC (
1:100
) (
MAB3138
, R&D Systems, Minneapolis, MN, United States), rat anti-CD3 (
1:100
) (
ab11089
,
Abcam
, Cambridge, United Kingdom), or anti-
F4/80
(
1:50
) (
MCA497
,
Bio Rad
, Hercules, CA, United States)
(18)
STAT3 is Activated by CTGF-mediated Tumor-stroma Cross Talk to Promote HCC Progression
Cellular and Molecular Gastroenterology and Hepatology
September 20, 2022
Yuki Makino, Hayato Hikita, Seiya Kato, Masaya Sugiyama, Minoru Shigekawa, Tatsuya Sakamoto, Yoichi Sasaki, Kazuhiro Murai, Sadatsugu Sakane, Takahiro Kodama,
et al.
2223021"
F4/80
,
Bio-Rad
,"Cat#
MCA497RT
, RRID:AB 1102558" CD68,
Abcam
,"Cat#
ab955
, RRID:AB 307338" CD3,
Abcam
,"Cat#
ab11089
, RRID:AB 2889189" PECAM1,
Abcam
,"Cat#
ab28364
, RRID:AB 726362"
PCNA
,
Cell Signaling Technology
,"Cat#
(19)
Ketogenic diet uncovers differential metabolic plasticity of brain cells
Science advances
September 16, 2022
Düking T, Spieth L, Berghoff SA, Piepkorn L, Schmidke AM, Mitkovski M, Kannaiyan N, Hosang L, Scholz P, Shaib AH,
et al
.
RRID:AB_887879), Vinculin (
Sigma-Aldrich
, catalog no.
V9131
, RRID:AB_477629),
CD3
(
Abcam
, catalog no.
ab11089
, RRID:AB_2889189),
CD3e
(
BioLegend
, catalog no.
100312
,
clone
145-2C11; RRID:AB_312677), CD4 (
Becton Dickinson
(20)
Integrated single-cell transcriptomic analyses reveal that GPNMB-high macrophages promote PN-MES transition and impede T cell activation in GBM
EBioMedicine
September 1, 2022
Xiong A, Zhang J, Chen Y, Zhang Y, Yang F.
AF2330
, RRID:AB_2112934), anti-Mac-3 (
1:100
,
BD
,
550292
, RRID:AB_393587) and anti-CD3 (
1:100
,
Abcam
,
ab11089
/
ab16669
, RRID:AB_2889189/AB_443425) overnight at 4 °C. After washing in PBS, sections were stained with
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Anti-CD3 antibody [CD3-12] from Abcam
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